Composite

Part:BBa_K3258017:Design

Designed by: Alec Lourenco   Group: iGEM19_Stanford   (2019-10-16)


Self-amplifying fluorescent RNA (MDV-Q-beta-Spinach)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 180
    Illegal XbaI site found at 2231
    Illegal SpeI site found at 2107
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 146
    Illegal SpeI site found at 2107
    Illegal NotI site found at 2213
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 172
    Illegal BglII site found at 2228
    Illegal BamHI site found at 1538
    Illegal BamHI site found at 1681
    Illegal XhoI site found at 168
    Illegal XhoI site found at 1212
    Illegal XhoI site found at 2222
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 180
    Illegal XbaI site found at 2231
    Illegal SpeI site found at 2107
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 180
    Illegal XbaI site found at 2231
    Illegal SpeI site found at 2107
    Illegal NgoMIV site found at 2120
    Illegal NgoMIV site found at 2149
    Illegal AgeI site found at 1694
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

Q-beta replicase comes from Q-beta coliphage and E. coli. Spinach aptamer and MDV regions are a synthetic RNAs.

References